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自由基/活性氧分析

众多化学和生化反应过程都涉及自由基的参与,研究自由基的产生、结构及化学反应性能至关重要,目前形成了“自由基化学”。此外,现代生物学认为细胞呼吸获取氧份,多余的氧会转化成氧自由基。由于这种物质非常活跃,几乎可以与各种物质发生作用,引起一系列对细胞具有破坏性的连锁反应,从而损坏人体正常细胞和组织,引起多种疾病,这是人类衰老和患病的根源。另一方面,活性氧含量与生理过程密切相关,特别是许多病变过程都伴随着活性氧含量水平的明显异常变化。因此活性氧研究成为了生命科学的一研究热点课题。

   自由基/活性氧具有很高的反应活性,发展灵敏、准确的自由基或活性氧检测方法引起了广大科研工作者的兴趣。与其它自由基/活性氧测定方法相比,化学发光除具有条件温和、仪器简单、操作方便、灵敏度高等优点外,还具有发光现象与化学反应同时发生的特点,因此将化学发光的瞬时性与自由基短寿命相结合具有很好的应用前景。BPCL超微弱发光测量仪以其优异的灵敏度和良好的稳定性,在自由基/活性氧分析上具有重要应用,其例子有:

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[1]       Zhu B Z, Mao L, Huang C H, Fan R M, Kalyanaraman B, Zhu J G. Unprecedented hydroxyl radical-dependent two-step chemiluminescence production by polyhalogenated quinoid carcinogens and H2O2. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(40): 16046-16051.

[2]       Wang D B, Zhao L X, Guo L H, et al. Online detection of reactive oxygen species in ultraviolet (UV)-irradiated nano-TiO2 suspensions by continuous flow chemiluminescence. Analytical Chemistry, 2014, 86(21): 10535-10539.

[3]       Zou F J, Zhou W J, Guan W J, Lu C, Tang B Z. Screening of photosensitizers by chemiluminescence monitoring of formation dynamics of singlet oxygen during photodynamic therapy. Analytical Chemistry, 2016, 88(19): 9707-9713.

[4]       Zhu Y S, Li M, Wang X H, Jin H J, Liu S S, Xu J X, Chen Q. Caspase cleavage of cytochrome c1 disrupts mitochondrial function and enhances cytochrome c release. Cell Research, 2012, 22(1): 127-141.

[5]       Zhang G M, J, Liu G. Effect of diphenyl dimethyl bicarboxylate on concanavalin A-induced liver injury in mice. Liver International, 2005, 25(4): 904-912.

[6]       Guo S H, Bezard E, Zhao B L. Protective effect of green tea polyphenols on the SH-SY5Y cells against 6-OHDA induced apoptosis through ROS-NO pathway. Free Radical Biology & Medicine, 2005, 39(5): 682-695.

[7]       Lin Y, Jia R, Liu Y, et al. Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways. Free Radical Research, 2014, 48(12): 1485-1493.

[8]       Quan Y, Jiang C T, Xue B, Zhu S G, Wang X. High glucose stimulates TNFα and MCP-1 expression in rat microglia via ROS and NF-κB pathways. Acta Pharmacologica Sinica, 2011, 32(2): 188-193.

[9]       Chang L, Zhang Z M, Li W J, Dai J, Guan Y F, Wang X. Liver-X-receptor activator prevents homocysteine-induced production of IgG antibodies from murine B lymphocytes via the ROS-NF-κB pathway. Biochemical & Biophysical Research Communications, 2007, 357(3): 772-778.

[10]    Chen A, Cao E T, Qin J F Qin J F. Arsenite-induced reactive oxygen species and the repression of α-tocopherol in the MGC-803 cells. European Journal of Pharmacology, 2002, 448(1): 11-18.

[11]    Zhou W J, Cao Y Q, Sui D D, Lu C. Radical pair-driven luminescence of quantum dots for specific detection of peroxynitrite in living cells. Analytical Chemistry, 2016,88(5): 2659-2665.

[12]    Lin J M, Liu M L. Singlet oxygen generated from the decomposition of peroxymonocarbonate and its observation with chemiluminescence method. Spectrochimica Acta Part A Molecular & Biomolecular Spectroscopy, 2009,72(1):126-132.


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